Resistance against aqueous extract was observed based on the lowest zone of inhibition (28mm) and DMSO as the negative control. The antibacterial assay showed a high zone of inhibition (ZI) for both methanolic extract (>23mm, >22mm and >20mm) and ethanolic extract (>22mm, >22mm and >17mm) for P.aeruginosa, S.aureus and S.epidermis respectively. Thin layer chromatography revealed a high Rf value for flavonoids (0.75mm) and alkaloids (0.60mm). The result of the qualitative phytochemical screening showed the presence of flavonoids and alkaloids. The excision bioassay analysis was used in examining the wound healing process in mice. The effect of methanolic, ethanolic and aqueous extracts of Tillandsia usneoides against pathogenic bacteria were evaluated on antimicrobial activities using disc diffusion and broth dilution susceptibility assay. Physical and chemical evaluations were done through phytochemical screening and thin layer chromatography. This research was designed to explore the antimicrobial efficacy of Tillandsia usneoides against skin infections in wound healing. 1600x2400px yellow, phalaenopsis orchid flowers, bud, moth orchids, blooming, greenhouse, winter.Wound healing is a dynamic phenomenon that results in the restoration of anatomic continuity and function on which can be delayed by pathogenic bacteria.1600x2400px yellow, phalaenopsis orchid flowers, moth orchids, blooming, greenhouse, winter, surrounded, spanish mosses, mosses., yellow phalaenopsis orchid Public Domain.
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